Molecular Genetics Notes for M.Sc. Biotechnology | HNBGU Approved Syllabus | Chromatin, Mutations, Transposons & Gene Transfer
M.Sc. Biotechnology III Sem.
Paper-I Molecular Genetics
(Course Code: SOLS/MBT/DSC-7)
No. of Credits: 5
UNIT – I (Cr- 1)
Chromatin: Structure of chromatin and higher-order packaging in the chromosomes.
Principles of Genetic Inheritance: Mendelian law of Inheritance and its variations, linkage and crossing over, gene mapping, recombination, complementation, and extrachromosomal inheritance.
UNIT – II (Cr- 1)
Mutations: Types, mutation rate, reversion vs. suppression, mutagenic agents, molecular mechanism of mutagenesis.
Chromosomal aberrations: Changes in number and structure.
Ploidy: Types & genetic implications.
UNIT- III (Cr- 1)
Transposons: Transposable genetic elements, insertion sequences, composite and complex transposons, replicative and non-replicative transposition, and genetic analysis using transposons.
UNIT–IV (Cr- 1)
Bacterial Conjugation: F-factor, conjugal transfer process, high frequency recombination (Hfr)
strains.
Bacterial transformation: Competence, DNA uptake by competent cells, and mechanism of
transformation.
UNIT-V (Cr- 1)
Transduction: General & specialized transduction.
Genetics of bacteriophages: Lytic and lysogenic cycles, expression of phage genes in the regulation of lytic and lysogenic circuits.
Recommended Books:
1. Shergojry, S.A., Nisham, M.U. Introduction to Molecular Genetics. 2024. 1st ed. Lambert Academic Publisher.
2. Bhat, T.A., Shakoori, R., Al-Khayri, J.M. Molecular Genetics, Structure, Mechanism, and Functions. 2024. 1st ed. CRC Press.
3. Andres, A., Parafianowicz, P., Lantz, K. Molecular Genetics. 2021. 1st ed. Kendall/Hunt Publishing Company.
4. Snustad, D.P., Simmons, M.J. Principles of Genetics. 2015. 7th ed. John Wiley & Sons.
5. Joshi, D. Molecular Genetics of Bacteria. 2011. 1st ed. Oxford Book Company
6. Maloy, S.R., Cronan, J.E., Freifelder, D. Microbial Genetics. 1994. 2nd ed. Jones and Bartlett Publishers, Inc.
For Microbial Physiology click on Microbial Physiology and Metabolism Lecture Notes