Welcome to the Genomics and Proteomics Lecture Notes section for M.Sc. Microbiology (III Semester, HNBGU). These comprehensive, exam-oriented notes cover genome organization, genome mapping, sequencing technologies, functional genomics, transcriptomics, proteomics, bioinformatics, and their applications in modern biological research.
Genomics and Proteomics Lecture Notes | M.Sc. Microbiology (HNBGU)
III SEMESTER SLS/MIC/DSC-9: GENOMICS AND PROTEOMICS
Unit I
Chapter 1: Genome Anatomies
Chapter 2: Introduction to structural, comparative, and functional genomics;
Chapter 3: Applications of genomics
Chapter 4: Anatomy of eukaryotic and prokaryotic genome.
Chapter 5: Genome size and complexity;
Chapter 6: Repetitive DNA content of genome;
Chapter 7: Introduction to gene networks and epigenetic analysis;
Chapter 8: DNA methylation analysis:
Chapter 9: Global DNA methylation analysis
Chapter 10: Gene-specific methylation analysis,
Chapter 11: Methylation sensitive PCR,
Chapter 12: Quantitative Methods of DNA methylation analysis
Chapter13: Sequencing of genome:
Chapter 14: Shot gun sequencing,
Chapter 15: High throughput sequencing;
Chapter 16: Methods for sequence assembly:
Chapter 17: Whole genome shot gun approach,
Chapter 18: Clone contig approach.
Unit II
Chapter 20: Genetic Mapping: DNA Markers Used (RFLP, SSLP and SNP)
Chapter 21: Gene Mapping by Linkage and Pedigree Analysis
Chapter 22: Genetic Mapping in Bacteria
Chapter 23: Limitations of Genetic Mapping
Chapter 24: Physical Mapping of Genomes
Chapter 25: Restriction Mapping
Chapter 26: Fluorescence In Situ Hybridization (FISH)
Chapter 27: Sequence Tagged Site (STS) Mapping
Chapter 28: Genome Sequence Analysis
Unit III
Chapter 29: Location of Gene by Sequence Inspection
Chapter 30: Techniques Used for Gene Location
Chapter 31: Northern Hybridization
Chapter 34: Techniques used for transcript mapping (RACE and heteroduplex analysis)
Chapter 35: Location of Exons and Exon–Intron Boundaries
Chapter 36: Determining Function of Individual Genes
Chapter 38: Gene Inactivation by Homologous Recombination (Gene Targeting and Gene Trapping)
Chapter 39: Genome-Wide Mutagenesis
Chapter 40: Transposon Tagging
Chapter 41: RNA Interference (RNAi)
Chapter 42: Overexpression of Genes
Chapter 43: Directed Mutagenesis
Chapter 44: Determining Pattern of Gene Expression
Chapter 45: Reporter Gene and Immunocytochemistry
Chapter 46: Human Genome Project (HGP): Strategies and Implications
Unit IV
Chapter 48: Serial Analysis of Gene Expression (SAGE)
Chapter 49: Massively Parallel Signature Sequencing (MPSS)
Chapter 50: DNA Chip and Microarray
Chapter 52: Applications of Transcriptomics
Unit V
Chapter 53: