Recombinant DNA Technology PPTs
Introduction to Recombinant DNA technology and Gene Cloning
Restriction endonucleases and DNA modifying enzymes (ligase, polymerases, alkaline phosphatase, polynucleotide kinase, terminal transferase, Reverse transcriptase)
Phages: M13 phage vectors phagmid PPT
Lambda phage vectors cosmid PPT
Yeast cloning vectors, Cloning vectors for plants and animals. Artificial chromosomes BAC, PAC & YAC.
Isolation of nucleic acid from bacteria, animal, plants, plasmid DNA and Phage DNA.
Basic steps of gene cloning
Cloning strategies (cell based cloning and PCR based gene cloning
Genomic library
Synthesis of cDNA. Construction of cDNA library
Selection of r DNA clones and their expression products, Chromosome walking.
Expression of cloned genes in heterologous host.
DNA sequencing: Chemical and enzymatic methods. 0.5
PCR: Types and applications. Real Time PCR. Site directed mutagenesis. Ribonuclease protection assay, Gel retardation assay,
DNA foot printing, DNA finger printing, DNA profiling.
Genomic analysis: S-1 mapping, RFLP, RAPD, AFLP. 0.5
Probe labeling and hybridization. Blotting techniques: Southern, Northern and Western blotting (Methodologies and applications)
Transgenic Technology: Types, approaches & applications (Plant & Animals), 0.5
Gene therapy: Principles, strategies and ethics of gene therapy. Genome editing technologies: Principles and applications.