Recombinant DNA Technology PPTs
Yeast cloning vectors,
Animal and plant viruses as vectors. Artificial chromosomes
BAC, PAC & YAC.
Isolation of nucleic acid from bacteria (plasmid and genomic DNA), Plant, animal & bacteria.
Basic steps of gene cloning
Cloning strategies (cell based cloning and PCR based gene clonoing
Synthesis of cDNA. Construction of cDNA library
Selection of r DNA clones and their expression products, Chromosome walking.
Expression of cloned genes in heterologous host.
DNA sequencing: Chemical and enzymatic methods. 0.5
PCR: Types and applications. Real Time PCR. Site directed mutagenesis. Ribonuclease protection assay, Gel retardation assay,
DNA foot printing, DNA finger printing, DNA profiling.
Genomic analysis: S-1 mapping, RFLP, RAPD, AFLP. 0.5
Probe labeling and hybridization. Blotting techniques: Southern, Northern and Western blotting (Methodologies and applications)
Transgenic Technology: Types, approaches & applications (Plant & Animals), 0.5
Gene therapy: Principles, strategies and ethics of gene therapy. Genome editing technologies: Principles and applications.