The page provides PPTs on various topics of Biochemistry
Introduction to Biochemistry and Historical perspectives
- Proteins:
- Amino acids Structure and Functional Group Properties,
- Amino acid Physical and Chemical Properties PPT
- Protein classification PPT
- Protein Structure I: Properties of Peptide bond, Ramachandran plot, Primary and secondary (alfa-helix, beta-pleated sheet, turns and loops) structures
- Protein Structure II: Tertiary and quaternary structures of proteins; Forces stabilizing tertiary structure
- Carbohydrates: Classification, Structures, Properties and functions of monosaccharides, polysaccharides, heteropolysaccharides
- Lipids: Classification, nomenclature, general properties and functions
- Nucleic acid structure I: DNA structure, different form of DNA
- Nucleic acid structure II: RNA Structure and types
Bioenergetics
- Bioenergetics I: Concept of free energy
- Bioenergetics II: Biological oxidation reduction
- Bioenergetics III: High Energy Compounds
Enzymes
- Enzyme Purification
- Introduction to enzymes I: Properties, Active site, Nomenclature and Classification
- Cofactors: Coenzymes and metal ions
- Factors affecting enzyme activity
- Enzyme II Mechanism of enzyme action: ES complex, transition state, activation energy, binding energy, Hypothesis of enzyme action: Key and Lock theory; Induced fit theory;Classes of enzyme specificity
- Enzyme III Progress curve, Michaelis Menten derivation, Determination of Vmax and Km, Catalytic efficiency (Kcat/Km) Lineweaver-Burk plot, Eadie-Hofstee Plot, Bi-substrate reactions (Sequential and double-displacement reaction) Effects of Temperature and pH on enzyme activities
- Multifunctional enzyme Fatty Acid synthase
- Enzyme V(II) Catalytic Mechanisms II: Metal Ion Catalysis; Catalysis By Approximation, Strain And Distortion
- Regulatory Strategies I Allosteric Control of enzymes Feedback Inhibition; Properties of Allosteric control; Models for Allosteric regulation: The Symmetry Model (MWC model); The Sequential Model (KNF model); Allosteric regulation of Aspartate Transcarbomylase (ATCase); Different forms of enzymes (Isozymes/Isoenzymes) Lactate dehydrogenase)
- Regulatory Strategies II: Proteolytic Activation (Zymogen Activation), Digestive Proteases: Fibrinogen And Prothrombin; Reversible Covalent Modification: (Phosphorylation With Special Reference To Glycogen Phosphorylase)
- Intermediary metabolism
- Carbohydrate metabolism and its regulation
- Carbohydrates Breakdown I: Glycolysis; Rate of glycolysis in the absence of O2 (Pasture effect ); Rate of glycolysis in the cancerous cells Fate of Pyruvate: Lactic acid fermentation; Alcoholic fermentation; Feeder pathway of glycolysis; Galactose breakdown and metabolic disturbances; Regulation of glycolysis (PFK, HK and PyrK), Glucose transporter and their regulation by Insulin
- Carbohydrate Metabolism II: Citric acid cycle (Krebs cycle) and Anaplerotic reactions
- Gluconeogenesis: Reactions and the Key enzymes of gluconeogenesis Regulation of gluconeogenesis and Cori cycle
- Glyoxylate cycle and its regulation
- Pentose Phosphate Pathway Overview, Oxidative and non-oxidative phases Reactions and Key enzymes Regulation, Functions and Diseases of HMP Pathway
- Mitochondrial Oxidative Phosphorylation & Electron Transport Chain I: (Overview, Redox potentials and Standard free energy change of electron transfer, Electron carriers)
- Oxidative Phosphorylation II: Electron Transport Chain; Respiratory complexes: order and organization, Proton motive force, Chemiosmotic theory
- Oxidative Phosphorylation III: ATP synthase: Structure, Mechanism of ATP synthesis, P/O ration
- Oxidative Phosphorylation V: Inhibitors and Uncouplers
- Glycogen Metabolism: Glycogenolysis and Glycogenesis and their regulation, Hormonal Regulation of Carbohydrate Metabolism
- Ketogenesis: Synthesis of ketone bodies
- Fermentation in different microorganism
- Gamma aminobutyrate shunt pathway
- Entner-Doudoroff pathway,
- Glucuronate pathway
Amino metabolism
- General reactions of amino acid metabolism: Transamination, Oxidative and Non-oxidative Deamination and Decarboxylation
- Overview of Amino Acid degradation
- Urea cycle, Linkage between Urea cycle & Citric Acid cycle, Regulation of Urea cycle
- Overview of amino acid biosynthesis
- Inborn errors of Amino acid catabolic pathways
- Biochemistry of Biological Nitrogen Fixation
- Biosynthesis of porphyrins
- Lipid Metabolism and its regulation
- Fat breakdown: Hydrolysis of dietary triacylglycerol, Fatty acid breakdown, Role of carnitine, ß-oxidation of fatty acids, Oxidation of unsaturated fatty acids, Oxidation of odd numbered fatty acids, Fate of propionate, Regulation of fatty acid metabolism, β-oxidation in Peroxisome, α-and ω- oxidation of fatty acids
- Ketogenesis: Synthesis of ketone bodies
- Fatty acid biosynthesis: Acetyl CoA carboxylase, fatty acid synthase, ACP structure and function, Fatty acid synthesis, Regulation of fatty acid synthesis, Long chain fatty acid synthesis, Desaturation of saturated fatty acid
- Biosynthesis of Eicosanoids: Prostaglandins (PG) Thromboxanes (TX) Leukotrienes (LT) and Lipoxins (LX)
- Biosynthesis of Triacylglycerols, Membrane phospholipids and prostaglandins
- Cholesterol biosynthesis and its regulation
- Lipoproteins (Chylomicrons, VLDL, LDL & HDL); Defects in cholesterol metabolism
- Metabolic and Inborn Disorders of lipid metabolism
Nucleotide metabolism
- Biosynthesis of purine and pyrimidine nucleotides and their regulation, Purine salvage pathways, Catabolic pathways of purine and pyrimidine nucleotides
- Biosynthesis of deoxyribonucleotides, Role of ribonucleotide reductase, Synthesis of deoxythymidylate, Inhibitors of nucleic acid biosynthesis