Paper-V Omics Technology
(Course Code: SOLS/MBT/DSE-1a)
No. of Credits: 3
Unit I (Cr- 0.75)
Chapter I: Introduction to omics technologies and systems biology
Chapter 2: Genome organization
and comparative genomics, Historical background and evolution of epigenetics, Genome vs epigenome principles of epigenomics including DNA methylation, acetylation and chromatin remodelling and epigenetic memory.
Unit II (Cr- 0.75)
Introduction to proteomics and protein structure, protein extraction and separation techniques including SDS-PAGE and 2D electrophoresis, mass spectrometry techniques protein interactions.
Unit III (Cr- 0.75)
Concept of transcriptomics and gene expression analysis, coding and non-coding RNAs, transcriptomic techniques including RT-PCR, quantitative Real Time-PCR, and differential gene expression analysis.
Unit IV (Cr- 0.75)
Introduction to metabolomics and metabolic profiling, targeted and untargeted metabolomics approaches, analytical techniques and applications of metabolomics.
Recommended Books:
1. Brown T.A. Genomes 5. 2023. 5th ed. CRC Press.
2. Twyman R. Principles of Proteomics. 2014. 2nd ed. Taylor & Francis.
3. Primrose S.B., Twyman R. Principles of Genome Analysis and Genomics. 2009. 3rd ed. John Wiley & Sons.
4. Liebler D.C. Introduction to Proteomics: Tools for the New Biology. 2002. 1st ed. Humana Totowa, NJ.
Genomics and Proteomics Notes PDF | M.Sc. Microbiology HNBGU