Enzyme III​  Progress curve, Michaelis Menten derivation, Determination of Vmax and Km, Catalytic efficiency (Kcat/Km) Lineweaver-Burk plot, Eadie-Hofstee Plot, Bi-substrate reactions (Sequential and double-displacement reaction) ​  Effects of Temperature and pH on enzyme activities

Enzyme III​ Progress curve, Michaelis Menten derivation, Determination of Vmax and Km, Catalytic efficiency (Kcat/Km) Lineweaver-Burk plot, Eadie-Hofstee Plot, Bi-substrate reactions (Sequential and double-displacement reaction) ​ Effects of Temperature and pH on enzyme activities

February 25, 2022

Enzyme III:Progress curve, Michaelis Menten derivation, Determination of Vmax and Km, Catalytic efficiency (Kcat/Km) Lineweaver-Burk plot, Eadie-Hofstee Plot, Bi-substrate reactions (Sequential and double-displacement reaction),Effects of Temperature and pH on enzyme activities

  • In a enzyme catalyzed reaction, when product formed is plotted against the time duration is called progress curve.
  • In initial phase, as Incubation time ↑, the amount of product formed ↑and it become proportional to the  incubation times.
  • In later phase of the reaction the curve become sloppy as product formed does not ↑ further with ↑ in time.
  • It may be due to the exhaustion of substrate  or  accumulation of the product to the inhibitory levels or production of inhibitor(s) during the reaction

                          E+P      ↔        P

  • The rate of catalysis velocity (V0), is defined as the number of moles of product formed per second or minute.
  • Enzyme activity (V)= Product formed/incubation time 

9 responses to “Enzyme III​ Progress curve, Michaelis Menten derivation, Determination of Vmax and Km, Catalytic efficiency (Kcat/Km) Lineweaver-Burk plot, Eadie-Hofstee Plot, Bi-substrate reactions (Sequential and double-displacement reaction) ​ Effects of Temperature and pH on enzyme activities”

  1. Sonal says:

    Very good material

  2. Manoj says:

    Nicely explained, content is good….thank you

  3. Radhika Naudiyal says:

    Very informative presentation. Thoroughly explained and informative. Thank you for sharing this.

  4. Rakhi says:

    Amazing content..

  5. Nancy Bhatnagar says:

    Thank you for such concise notes.

  6. […] Enzyme III​ Progress curve, Michaelis Menten derivation, Determination of Vmax and Km, Catalytic e…​ […]

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