DNA Denaturation and Renaturation Kinetics

DNA Denaturation and Renaturation Kinetics

November 28, 2021
  • Denaturation is defined as the separation of the two complementary strands of duplex DNA. It is also called as melting of ds DNA.
  • Renaturation is defined as the reassociation of the two separated complementary strands of DNA.  It is also called as annealing
  • DNA  can be Denaturated by heat or use of alkali solution
  • Extent of DNA denaturation at any temperature can be measured by the change   in UV absorbance of DNA  solution
  • Double stranded DNA show less UV absorption in comparison to ssDNA  at 260 nm. Therefore, when DNA  is denatured  the UV absorption of the solution is increased by  30 to 40%. This is called Hyperchromism .
  • The UV absorption temperature profile of DNA is called melting curve in which, the  relative  UV absorption of DNA solution was plotted  against  temperature.
  • Melting Temperature: Temperature at which half of the DNA is denatured.  This is approximately  85O C  for most of the DNA.
  • It is the characteristic property of DNA

One response to “DNA Denaturation and Renaturation Kinetics”

  1. […] DNA Denaturation and Renaturation Kinetics […]

Leave a Reply

Your email address will not be published. Required fields are marked *