DNA Denaturation and Renaturation Kinetics
- Denaturation is defined as the separation of the two complementary strands of duplex DNA. It is also called as melting of ds DNA.
- Renaturation is defined as the reassociation of the two separated complementary strands of DNA. It is also called as annealing
- DNA can be Denaturated by heat or use of alkali solution
- Extent of DNA denaturation at any temperature can be measured by the change in UV absorbance of DNA solution
- Double stranded DNA show less UV absorption in comparison to ssDNA at 260 nm. Therefore, when DNA is denatured the UV absorption of the solution is increased by 30 to 40%. This is called Hyperchromism .
- The UV absorption temperature profile of DNA is called melting curve in which, the relative UV absorption of DNA solution was plotted against temperature.
- Melting Temperature: Temperature at which half of the DNA is denatured. This is approximately 85O C for most of the DNA.
- It is the characteristic property of DNA