Eukaryotic Transcription and Post-transcriptional modifications
Eukaryotic transcription and Post-transcriptional modification (5’capping and 3’ poly (A) tailing)
- Overall process of RNA synthesis in eukaryotes is quite complex.
- Transcription takes place in the nucleus.
- Transcription units in eukaryotes are mostly monogenic.
- Five different RNA polymerases are present each for the transcription of a specific class of genes.
- Eukaryotic RNA polymerases require the assistance of transcription factors in order to initiate the synthesis of RNA chains.
- Protein coding primary transcripts (mRNA) undergo three major post-transcriptional modifications prior to their transport to the cytoplasm for translation
- 5′ – 7-Methyl guanosine capping
- 3′ Poly(A) tailing
- If present, intron sequences are spliced out of transcripts
For details on prokaryotic transcription, click on following link