Lambda and M13 Phage DNA Isolation - Biotech MCQ

Lambda and M13 Phage DNA Isolation

PPTs, Recombinant DNA Technology

Lambda and M13 Phage DNA Isolation

Biotech Tutorials

March 21, 2024

The presentation provides detail on the steps of lambda and M13 DNA isolation. Lambda and M13 phage DNA are being used as cloning vectors. For detail on phage DNA as cloning vector click on following link Recombinant DNA Technology PPTs;

Lambda phage vectors cosmid PPT

M13 phage vectors phagmid PPT

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MCQs on Intermediate Filaments

MCQs on Intermediate Filaments

December 12, 2024

Intermediate Filaments PPT

Intermediate Filaments PPT

December 11, 2024

MCQs on Introduction to Recombinant DNA Technology and Gene Cloning

MCQs on Introduction to Recombinant DNA Technology and Gene Cloning

December 6, 2024

MCQs on Strcture and Functions of Actin filament cytoskeleton

MCQs on Strcture and Functions of Actin filament cytoskeleton

December 5, 2024

Cytoskeleton Microfilament Structure and Functions PPT

Cytoskeleton Microfilament Structure and Functions PPT

December 4, 2024

Structure and functions of Microtubule Cytoskeleton

Structure and functions of Microtubule Cytoskeleton

November 14, 2024

Structure and Functions of Chloroplast

Structure and Functions of Chloroplast

November 5, 2024

Structure and functions of Mitochondria

Structure and functions of Mitochondria

August 13, 2024

Structure and functions of nucleus

Structure and functions of nucleus

August 1, 2024

Structure and functions of plant vacuoles

Structure and functions of plant vacuoles

July 25, 2024

Structure and functions of Golgi body

Structure and functions of Golgi body

July 16, 2024

Endoplasmic Reticulum

Endoplasmic Reticulum

July 12, 2024

Peroxisomes and Glyoxisomes

Peroxisomes and Glyoxisomes

July 11, 2024

Structure and function of endosome and lysosome

Structure and function of endosome and lysosome

July 10, 2024

Fermentation in microorganisms

Fermentation in microorganisms

June 11, 2024

Factors affecting enzyme activity

Factors affecting enzyme activity

April 7, 2024

Plasmid DNA isolation

Plasmid DNA isolation

March 20, 2024

Multifunctional enzyme Fatty Acid synthase

Multifunctional enzyme Fatty Acid synthase

March 13, 2024

Ribozymes PPT

March 12, 2024

Genomic DNA Isolation from bacteria Animal & Plant Tissues

Genomic DNA Isolation from bacteria Animal & Plant Tissues

March 6, 2024

Artificial Chromosomes: PACs BACs YACs

Artificial Chromosomes: PACs BACs YACs

March 4, 2024

Cloning vectors for plants and animals

Cloning vectors for plants and animals

February 21, 2024

Yeast DNA vectors PPT

Yeast DNA vectors PPT

February 21, 2024

Lambda phage vectors cosmid PPT

Lambda phage vectors cosmid PPT

February 21, 2024

M13 phage vectors phagmid PPT

M13 phage vectors phagmid PPT

February 21, 2024

MCQs on Restriction Endonucleases

MCQs on Restriction Endonucleases

February 20, 2024

Energetics of Transport

Energetics of Transport

January 21, 2024

Ion Channels

January 20, 2024

Biomembranes

January 17, 2024

Secondary active transport

Secondary active transport

January 15, 2024

Active Transport by ATP-driven pumps

Active Transport by ATP-driven pumps

January 13, 2024

Simple and facilitated diffusion across cell membrane

Simple and facilitated diffusion across cell membrane

January 6, 2024

Metabolic Homeostasis: Regulation of Body mass and Eating behavior

Metabolic Homeostasis: Regulation of Body mass and Eating behavior

December 11, 2023

Integration of metabolism: Hormonal integration of fuel Metabolism

Integration of metabolism: Hormonal integration of fuel Metabolism

December 6, 2023

Integration of metabolism: Tissue specific metabolism

Integration of metabolism: Tissue specific metabolism

December 6, 2023

Porphyrias

October 27, 2023

Biosynthesis and degradation of Porphyrins

Biosynthesis and degradation of Porphyrins

October 26, 2023

Membrane Rafts

October 20, 2023

Cell Fusion

October 19, 2023

Fluorescence Recovery After Photobleaching (FRAP) Analysis

Fluorescence Recovery After Photobleaching (FRAP) Analysis

October 19, 2023

Membrane Dynamics and Fluidity

Membrane Dynamics and Fluidity

October 11, 2023

Composition Structure Functions of Plasma Membrane

Composition Structure Functions of Plasma Membrane

October 9, 2023

Introduction to cell and cell biology

Introduction to cell and cell biology

October 4, 2023

What is Biotechnology?

What is Biotechnology?

October 1, 2023

Plasmid vectors

Plasmid vectors

June 18, 2023

DNA modifying enzymes (ligase, polymerases, alkaline phosphatase, polynucleotide kinase, terminal transferase, Reverse transcriptase)

DNA modifying enzymes (ligase, polymerases, alkaline phosphatase, polynucleotide kinase, terminal transferase, Reverse transcriptase)

June 18, 2023

Restriction endonucleases

Restriction endonucleases

June 18, 2023

Introduction to Recombinant DNA technology and Gene Cloning

Introduction to Recombinant DNA technology and Gene Cloning

June 14, 2023

Metalloproteins in electron transport processes: cytochromes, ion-sulphur proteins and their Synthetic Models

Metalloproteins in electron transport processes: cytochromes, ion-sulphur proteins and their Synthetic Models

April 11, 2023

Electron transfer in biology

Electron transfer in biology

March 26, 2023

Metal complexes in transmission of energy

Metal complexes in transmission of energy

February 15, 2023

Enzyme Purification

Enzyme Purification

December 20, 2022

Ion transport across cell membrane

Ion transport across cell membrane

December 14, 2022

Standard free energy change in biological reactions, exergonic, endergonic

Standard free energy change in biological reactions, exergonic, endergonic

December 13, 2022

Prokaryotic and eukaryotic genes Split genes Pseudogenes Gene superfamily

Prokaryotic and eukaryotic genes Split genes Pseudogenes Gene superfamily

December 4, 2022

Structure and functions of cell membrane

Structure and functions of cell membrane

December 4, 2022

DNA in living system

DNA in living system

November 30, 2022

RNA in living organism

RNA in living organism

November 30, 2022

Helix-to-coil transition in DNA

Helix-to-coil transition in DNA

November 28, 2022

Biological cell and its constituents

Biological cell and its constituents

November 25, 2022

Bioenergetics of DNA polymerization and glucose storage

Bioenergetics of DNA polymerization and glucose storage

November 23, 2022

Glycogen storage diseases Pentosuria Galactose tolerance test and Galactosemia

Glycogen storage diseases Pentosuria Galactose tolerance test and Galactosemia

November 17, 2022

Glucose Homeostasis Diabetes mellitus Glucose tolerance Test

Glucose Homeostasis Diabetes mellitus Glucose tolerance Test

November 17, 2022

RNA editing

September 4, 2022

Biosynthesis of deoxyribonucleotides, Role of ribonucleotide reductase, Synthesis of deoxythymidylate, Inhibitors of nucleic acid biosynthesis

Biosynthesis of deoxyribonucleotides, Role of ribonucleotide reductase, Synthesis of deoxythymidylate, Inhibitors of nucleic acid biosynthesis

June 11, 2022

Biosynthesis of purine and pyrimidine nucleotides and their regulation, Purine salvage pathways, Catabolic pathways of purine and pyrimidine nucleotides

Biosynthesis of purine and pyrimidine nucleotides and their regulation, Purine salvage pathways, Catabolic pathways of purine and pyrimidine nucleotides

June 8, 2022

Biosynthesis of Eicosanoids: Prostaglandins (PG) Thromboxanes (TX) Leukotrienes (LT) and Lipoxins (LX)

Biosynthesis of Eicosanoids: Prostaglandins (PG) Thromboxanes (TX) Leukotrienes (LT) and Lipoxins (LX)

May 29, 2022

Fatty acid biosynthesis: Acetyl CoA carboxylase, fatty acid synthase, ACP structure and function, Fatty acid synthesis, Regulation of fatty acid synthesis, Long chain fatty acid synthesis, Desaturation of saturated fatty acid

Fatty acid biosynthesis: Acetyl CoA carboxylase, fatty acid synthase, ACP structure and function, Fatty acid synthesis, Regulation of fatty acid synthesis, Long chain fatty acid synthesis, Desaturation of saturated fatty acid

May 27, 2022

Inborn errors of Amino acid catabolic pathways

Inborn errors of Amino acid catabolic pathways

May 18, 2022

Lipoproteins (Chylomicrons, VLDL, LDL & HDL); Defects in cholesterol metabolism

Lipoproteins (Chylomicrons, VLDL, LDL & HDL); Defects in cholesterol metabolism

May 15, 2022

Cholesterol biosynthesis and its regulation

Cholesterol biosynthesis and its regulation

May 14, 2022

Biosynthesis of Triacylglycerols Phosphoglycerides and Sphingomyelin

Biosynthesis of Triacylglycerols Phosphoglycerides and Sphingomyelin

May 13, 2022

Overview of amino acid biosynthesis

Overview of amino acid biosynthesis

May 13, 2022

Biochemistry of Biological Nitrogen Fixation

Biochemistry of Biological Nitrogen Fixation

May 3, 2022

Overview of Amino Acid degradation

Overview of Amino Acid degradation

April 30, 2022

Urea cycle, Linkage between Urea cycle & Citric Acid cycle, Regulation of Urea cycle

Urea cycle, Linkage between Urea cycle & Citric Acid cycle, Regulation of Urea cycle

April 30, 2022

General reactions of amino acid metabolism: Transamination, Oxidative and Non-oxidative Deamination and Decarboxylation

General reactions of amino acid metabolism: Transamination, Oxidative and Non-oxidative Deamination and Decarboxylation

April 29, 2022

Gamma aminobutyrate shunt pathway

Gamma aminobutyrate shunt pathway

April 28, 2022

Uronic acid (Glucuronate) pathway

Uronic acid (Glucuronate) pathway

April 27, 2022

Entner-Doudoroff pathway

Entner-Doudoroff pathway

April 26, 2022

Fat breakdown: Hydrolysis of dietary triacylglycerol, Fatty acid breakdown, Role of carnitine, ß-oxidation of fatty acids, Oxidation of unsaturated fatty acids, Oxidation of odd numbered fatty acids, Fate of propionate, Regulation of fatty acid metabolism, β-oxidation in Peroxisome, α-and ω- oxidation of fatty acids

Fat breakdown: Hydrolysis of dietary triacylglycerol, Fatty acid breakdown, Role of carnitine, ß-oxidation of fatty acids, Oxidation of unsaturated fatty acids, Oxidation of odd numbered fatty acids, Fate of propionate, Regulation of fatty acid metabolism, β-oxidation in Peroxisome, α-and ω- oxidation of fatty acids

April 15, 2022

Ketogenesis: Synthesis of ketone bodies

Ketogenesis: Synthesis of ketone bodies

April 13, 2022

Glycogen Metabolism: Glycogenolysis and Glycogenesis and their regulation, Regulation of Carbohydrate Metabolism

Glycogen Metabolism: Glycogenolysis and Glycogenesis and their regulation, Regulation of Carbohydrate Metabolism

April 11, 2022

Oxidative Phosphorylation V: Inhibitors and Uncouplers

Oxidative Phosphorylation V: Inhibitors and Uncouplers

April 8, 2022

Oxidative Phosphorylation IV: Transport of ADP, Pi and cytosolic electrons to Mitochondria; Regulation of oxidative phosphorylation

Oxidative Phosphorylation IV: Transport of ADP, Pi and cytosolic electrons to Mitochondria; Regulation of oxidative phosphorylation

April 5, 2022

Oxidative Phosphorylation III: ATP synthase, Structure and Mechanism of action ATP synthesis, P/O ratio

Oxidative Phosphorylation III: ATP synthase, Structure and Mechanism of action ATP synthesis, P/O ratio

April 4, 2022

Oxidative Phosphorylation II: Electron Transport Chain; Respiratory complexes: order and organization, Proton motive force, Chemiosmotic theory

Oxidative Phosphorylation II: Electron Transport Chain; Respiratory complexes: order and organization, Proton motive force, Chemiosmotic theory

April 3, 2022

Mitochondrial Oxidative Phosphorylation & Electron Transport Chain I: Overview, Redox potentials and Standard free energy change of electron transfer, Electron carriers

Mitochondrial Oxidative Phosphorylation & Electron Transport Chain I: Overview, Redox potentials and Standard free energy change of electron transfer, Electron carriers

April 1, 2022

Pentose Phosphate Pathway Overview, Oxidative and non-oxidative phases Reactions and Key enzymes Regulation, Functions and Diseases of HMP Pathway

Pentose Phosphate Pathway Overview, Oxidative and non-oxidative phases Reactions and Key enzymes Regulation, Functions and Diseases of HMP Pathway

March 27, 2022

Glyoxylate cycle and its regulation

Glyoxylate cycle and its regulation

March 25, 2022

Gluconeogenesis: Reactions and the Key enzymes of gluconeogenesis Regulation of gluconeogenesis and Cori cycle

Gluconeogenesis: Reactions and the Key enzymes of gluconeogenesis Regulation of gluconeogenesis and Cori cycle

March 24, 2022

Citric acid cycle, Its function in energy generation and providing precursors for biosynthetic pathways; Anaplerotic reactions

Citric acid cycle, Its function in energy generation and providing precursors for biosynthetic pathways; Anaplerotic reactions

March 18, 2022

Glycolysis: steps, Rate in the absence of O2 (Pasture effect ) and in cancerous cells, Lactic acid and Alcoholic fermentation, Feeder pathway of glycolysis, Galactose breakdown (Leloir pathway) and metabolic disturbances; Regulation of glycolysis, Glucose transporter and their regulation by Insulin

Glycolysis: steps, Rate in the absence of O2 (Pasture effect ) and in cancerous cells, Lactic acid and Alcoholic fermentation, Feeder pathway of glycolysis, Galactose breakdown (Leloir pathway) and metabolic disturbances; Regulation of glycolysis, Glucose transporter and their regulation by Insulin

March 14, 2022

Regulatory Strategies II: Zymogen activation and Reversible Covalent Modification

Regulatory Strategies II: Zymogen activation and Reversible Covalent Modification

March 11, 2022

Allosteric Control of enzymes and Isozymes/Isoenzymes Lactate dehydrogenase)

Allosteric Control of enzymes and Isozymes/Isoenzymes Lactate dehydrogenase)

March 9, 2022

Enzyme V(II) Catalytic Mechanisms II: Metal Ion catalysis; Catalysis by Approximation, Strain and distortion

Enzyme V(II) Catalytic Mechanisms II: Metal Ion catalysis; Catalysis by Approximation, Strain and distortion

March 7, 2022

Catalytic Mechanisms of enzymes: Acid and base catalysis; Covalent catalysis; Reaction Mechanism of RNase, Lysozyme and Chymotrypsin

Catalytic Mechanisms of enzymes: Acid and base catalysis; Covalent catalysis; Reaction Mechanism of RNase, Lysozyme and Chymotrypsin

March 2, 2022

Enzyme IV: Enzyme inhibition; Reversible (Competitive, Noncompetitive and Uncompetitive) and Irreversible inhibition of enzymes

Enzyme IV: Enzyme inhibition; Reversible (Competitive, Noncompetitive and Uncompetitive) and Irreversible inhibition of enzymes

February 26, 2022

Enzyme III Progress curve, Michaelis Menten derivation, Determination of Vmax and Km, Catalytic efficiency (Kcat/Km) Lineweaver-Burk plot, Eadie-Hofstee Plot, Bi-substrate reactions (Sequential and double-displacement reaction) Effects of Temperature and pH on enzyme activities

Enzyme III Progress curve, Michaelis Menten derivation, Determination of Vmax and Km, Catalytic efficiency (Kcat/Km) Lineweaver-Burk plot, Eadie-Hofstee Plot, Bi-substrate reactions (Sequential and double-displacement reaction) Effects of Temperature and pH on enzyme activities

February 25, 2022

Enzyme II Mechanism of enzyme action: ES complex, transition state, activation energy, binding energy, Hypothesis of enzyme action: Key and Lock theory; Induced fit theory Classes of enzyme specificity

Enzyme II Mechanism of enzyme action: ES complex, transition state, activation energy, binding energy, Hypothesis of enzyme action: Key and Lock theory; Induced fit theory Classes of enzyme specificity

February 25, 2022

Coenzymes, Cofactors and Metalloenzymes: Structures, Properties and Functions

Coenzymes, Cofactors and Metalloenzymes: Structures, Properties and Functions

February 21, 2022

Enzymes: Properties Composition Active site and Classification

Enzymes: Properties Composition Active site and Classification

February 20, 2022

Lytic and Lysogenic cycle of λ Bacteriophage and Regulation

Lytic and Lysogenic cycle of λ Bacteriophage and Regulation

February 18, 2022

Life cycle of M13 Bacteriophage

Life cycle of M13 Bacteriophage

February 18, 2022

Generalized and Specialized Transduction

Generalized and Specialized Transduction

February 18, 2022

Gene Transfer In Bacteria-II: Transformation

Gene Transfer In Bacteria-II: Transformation

February 18, 2022

Gene Transfer In Bacteria I: Conjugation Hfr Conjugation Sexduction

Gene Transfer In Bacteria I: Conjugation Hfr Conjugation Sexduction

February 18, 2022

Bacterial Plasmids Properties and Types

Bacterial Plasmids Properties and Types

February 18, 2022

Chromosomal Aberrations

Chromosomal Aberrations

February 18, 2022

Cytoplasmic Inheritance

Cytoplasmic Inheritance

February 18, 2022

Linkage Recombination and chromosome mapping

Linkage Recombination and chromosome mapping

February 18, 2022

Exceptions to Mendel’s laws

Exceptions to Mendel’s laws

February 17, 2022

Mendel’s Law and Chromosomal theory of heredity

Mendel’s Law and Chromosomal theory of heredity

February 17, 2022

Dosage compensation Mechanisms: X-Inactivation, X-Hyperactivation and X-Hypoactivation

Dosage compensation Mechanisms: X-Inactivation, X-Hyperactivation and X-Hypoactivation

February 17, 2022

Homologous Recombination (Holliday model and DNA double strand repair model) and Site Specific Recombination

Homologous Recombination (Holliday model and DNA double strand repair model) and Site Specific Recombination

February 17, 2022

Transposable elements in prokaryotes and eukaryotes. Mechanism of transposition

Transposable elements in prokaryotes and eukaryotes. Mechanism of transposition

February 17, 2022

Intermediary metabolism

Intermediary metabolism

January 8, 2022

Bioenergetics III: High Energy Compounds

Bioenergetics III: High Energy Compounds

January 7, 2022

Bioenergetics: Biological Oxidation Reduction

Bioenergetics: Biological Oxidation Reduction

January 7, 2022

Bioenergetics I: Concept of free energy

Bioenergetics I: Concept of free energy

January 7, 2022

Ribonucleic Acid RNA

Ribonucleic Acid RNA

January 7, 2022

DNA structure Different form of DNA

DNA structure Different form of DNA

January 7, 2022

Lipids Classification Nomenclature Properties and Functions

Lipids Classification Nomenclature Properties and Functions

January 5, 2022

Carbohydrates: Classification, Structures, Properties and functions of monosaccharides, polysaccharides, heteropolysaccharides

Carbohydrates: Classification, Structures, Properties and functions of monosaccharides, polysaccharides, heteropolysaccharides

January 5, 2022

Tertiary and quaternary structures of proteins Forces stabilizing tertiary structure

Tertiary and quaternary structures of proteins Forces stabilizing tertiary structure

January 4, 2022

Protein Structure I: Properties of Peptide bond, Ramachandran plot, Primary and secondary (alfa-helix, beta-pleated sheet, turns and loops) structures

Protein Structure I: Properties of Peptide bond, Ramachandran plot, Primary and secondary (alfa-helix, beta-pleated sheet, turns and loops) structures

January 4, 2022

DNA Repair Pathways

DNA Repair Pathways

January 3, 2022

Mutations: Types, Mechanisms, Mutagens and Ames test

Mutations: Types, Mechanisms, Mutagens and Ames test

January 3, 2022

Regulation of eukaryotic gene expression (Chromatin remodeling, DNA methylation, environmental and biological control, DNA binding motifs, activators, enhancers and insulators)

Regulation of eukaryotic gene expression (Chromatin remodeling, DNA methylation, environmental and biological control, DNA binding motifs, activators, enhancers and insulators)

January 2, 2022

Regulation of Prokaryotic gene expression

Regulation of Prokaryotic gene expression

January 2, 2022

Post translational modifications, Protein degradation and non-ribosomal protein synthesis

Post translational modifications, Protein degradation and non-ribosomal protein synthesis

January 2, 2022

Translation in Prokaryotes and Eukaryotes

Translation in Prokaryotes and Eukaryotes

January 2, 2022

Genetic code and Wobble Hypothesis

Genetic code and Wobble Hypothesis

January 2, 2022

RNA splicing

January 1, 2022

Reverse transcription

Reverse transcription

January 1, 2022

Eukaryotic Transcription and Post-transcriptional modifications

Eukaryotic Transcription and Post-transcriptional modifications

January 1, 2022

Transcription in Prokaryotes

Transcription in Prokaryotes

January 1, 2022

DNA replication in eukaryotes

DNA replication in eukaryotes

December 1, 2021

DNA replication in prokaryotes

DNA replication in prokaryotes

December 1, 2021

Giant Chromosomes: Polytene Chromosome and Lampbrush Chromosome

Giant Chromosomes: Polytene Chromosome and Lampbrush Chromosome

November 28, 2021

Telomeres and centromere

Telomeres and centromere

November 28, 2021

DNA packaging into chromosome

DNA packaging into chromosome

November 28, 2021

C values and C value paradox

C values and C value paradox

November 28, 2021

DNA Denaturation and Renaturation Kinetics

DNA Denaturation and Renaturation Kinetics

November 28, 2021

Topological properties of DNA

Topological properties of DNA

November 28, 2021

Weak Acid-base Henderson-Hasselbalch equation

Weak Acid-base Henderson-Hasselbalch equation

October 25, 2021

Structure, properties and various forms of DNA

Structure, properties and various forms of DNA

October 24, 2021

Experimental evidences for nucleic acids as genetic material

Experimental evidences for nucleic acids as genetic material

October 24, 2021

Amino acid Physical and Chemical Properties

Amino acid Physical and Chemical Properties

October 19, 2021

Protein classification PPT

Protein classification PPT

October 17, 2021

Structure of Amino acids

Structure of Amino acids

October 14, 2021

Introduction to Biochemistry

Introduction to Biochemistry

October 11, 2021

Introduction to Biochemistry

Introduction to Biochemistry

October 11, 2021

Introduction to Biochemistry and Historical perspectives

Introduction to Biochemistry and Historical perspectives

October 10, 2021

Introduction to Molecular Biology

Introduction to Molecular Biology

October 10, 2021